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KD-Validated Anti-Caspase 8 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2349
Application
WB, FC, ICC
Uniprot Id
Q14790
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Caspase 8 antibody (Cat#ABG2349). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 8 antibody (Cat#ABG2349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Caspase-8 antibody (Cat#ABG2349). Caspase-8 expression in wild type (WT) and Caspase-8 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Caspase-8 antibody (Cat#ABG2349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Caspase 8 beta expression in HeLa cells using Caspase 8 beta antibody (Cat#ABG2349, 1:2,000). Green, isotype control; red, Caspase 8.
  • Immunocytochemical staining of HepG2 cells with anti-caspase 8 antibody (Cat#ABG2349, 1:1,000). Nuclei were stained blue with DAPI; Caspase 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2349-100 3 days 100 µL $499.00
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ABG2349-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Caspase 8 Rabbit Monoclonal Antibody
Accession (Primary)Q14790
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1665
Immunogen
A synthesized peptide derived from human Caspase-8
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 55 kDa , observed, 55 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q14790 )

Gene NameCASP8
Other Gene Names
Caspase 8; FLICE; MCH5; MACH; Casp-8; Caspase 8, Apoptosis-Related Cysteine Peptidase; Caspase 8, Apoptosis-Related Cysteine Protease; MORT1-Associated Ced-3 Homolog; ICE-Like Apoptotic Protease 5; Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; FADD-Like ICE; Caspase-8; CAP4; FADD-Homologous ICE/CED-3-Like Protease; FADD-Homologous ICE/Ced-3-Like Protease; MACH-Beta-1/2/3/4 Protein; MACH-Alpha-1/2/3 Protein; EC 3.4.22.61; ALPS2B; CASP-8
About Gene Symbol
CASP8 (Caspase 8) is a cysteine protease (caspase) involved in apoptosis. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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