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KD-Validated Anti-Carbonic anhydrase 9 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2352
Application
WB, ICC
Uniprot Id
Q16790
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Carbonic anhydrase 9 antibody (Cat#ABG2352). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Carbonic anhydrase 9 antibody (Cat#ABG2352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Carbonic anhydrase 9 antibody (Cat#ABG2352). Carbonic anhydrase 9 expression in wild type (WT) and Carbonic anhydrase 9 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Carbonic anhydrase 9 antibody (Cat#ABG2352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with CA9 antibody (Cat#ABG2352, 1:1,000). Nuclei were stained blue with DAPI; CA9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2352-100 3 days 100 µL $499.00
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ABG2352-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Carbonic anhydrase 9 Rabbit Monoclonal Antibody
Accession (Primary)Q16790
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1770
Immunogen
A synthesized peptide derived from human CA9
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 50 kDa , observed, 54 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q16790 )

Gene NameCA9
Other Gene Names
CA9; Carbonic Anhydrase 9; CAIX; MN; Carbonic Anhydrase IX; Renal Cell Carcinoma-Associated Antigen G250; RCC-Associated Protein G250; RCC-Associated Antigen G250; Carbonate Dehydratase IX; Carbonic Dehydratase; Membrane Antigen MN; P54/58N; CA-IX; PMW1; EC 4.2.1.1; G250
About Gene Symbol
CA9 (Carbonic Anhydrase 9) is a metabolic enzyme involved in adaptive immunity. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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