KD-Validated Anti-Cyclin dependent kinase inhibitor 1B Rabbit Monoclonal Antibody

Western blotting analysis using anti-Cyclin dependent kinase inhibitor 1B antibody (Cat#ABG2355). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin dependent kinase inhibitor 1B antibody (Cat#ABG2355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Cyclin dependent kinase inhibitor 1B antibody (Cat#ABG2355). Cyclin dependent kinase inhibitor 1B expression in wild type (WT) and cyclin dependent kinase inhibitor 1B shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin dependent kinase inhibitor 1B antibody (Cat#ABG2355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Cyclin dependent kinase inhibitor 1B expression in HAP-1 cells using Cyclin dependent kinase inhibitor 1B antibody (Cat#ABG2355, 1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 1B.

Immunocytochemical staining of HAP-1 cells with Cyclin dependent kinase inhibitor 1B antibody (Cat#ABG2355, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.