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KD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2356
Application
WB, FC, ICC
Uniprot Id
Q13111
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-p150 CAF1 antibody (Cat#ABG2356). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p150 CAF1 antibody (Cat#ABG2356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-p150 CAF1 antibody (Cat#ABG2356). p150 CAF1 expression in wild type (WT) and p150 CAF1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-p150 CAF1 antibody (Cat#ABG2356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of p150 CAF1 expression in HepG2 cells using p150 CAF1 antibody (Cat#ABG2356, 1:2,000). Green, isotype control; red, p150 CAF1.
  • Immunocytochemical staining of HepG2 cells with p150 CAF1 antibody (Cat#ABG2356, 1:1,000). Nuclei were stained blue with DAPI; p150 CAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Midum. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2356-100 3 days 100 µL $499.00
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ABG2356-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody
Accession (Primary)Q13111
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB755
Immunogen
A synthesized peptide derived from human integrin p150 CAF1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 107 kDa; Observed, 150 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q13111 )

Gene NameCHAF1A
Other Gene Names
CAF1P150; CAF1B; CAF-1; CAF1; P150; Chromatin Assembly Factor I P150 Subunit; Chromatin Assembly Factor I (150 KDa); CAF-I 150 KDa Subunit; CAF-1 Subunit A; CAF-I P150; MGC71229; Hp150; Chromatin Assembly Factor 1, Subunit A (P150); CTB-50L17.7; CAF
About Gene Symbol
CHAF1A (Chromatin Assembly Factor I P150 Subunit) is an epigenetic and nuclear regulatory protein involved in chromatin remodeling and epigenetics. It is an important research target in epigenetic regulation, chromatin biology, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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