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KD-Validated Anti-DLAT Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2363
Application
WB, FC, ICC
Uniprot Id
P10515
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-DLAT antibody (Cat#ABG2363). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DLAT antibody (Cat#ABG2363, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-DLAT antibody (Cat#ABG2363). DLAT expression in wild type (WT) and DLAT shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-DLAT antibody (Cat#ABG2363, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of DLAT expression in HepG2 cells using DLAT antibody (Cat#ABG2363, 1:2,000). Green, isotype control; red, DLAT.
  • Immunocytochemical staining of HepG2 cells with DLAT antibody (Cat#ABG2363, 1:1,000). Nuclei were stained blue with DAPI; DLAT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2363-100 3 days 100 µL $499.00
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ABG2363-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-DLAT Rabbit Monoclonal Antibody
Accession (Primary)P10515
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB805
Immunogen
A synthesized peptide derived from human DLAT
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 69 kDa ; Observed, 71 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P10515 )

Gene NameDLAT
Other Gene Names
DLAT; Dihydrolipoamide S-Acetyltransferase; PDC-E2; DLTA; E2; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate; Dehydrogenase Complex, Mitochondrial; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; E2 Component Of Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex Component E2; M2 Antigen Complex 70 KDa Subunit; EC 2.3.1.12; PDCE2; PBC; Dihydrolipoyllysine-Residue Acetyltransferase; EC 2.3.1
About Gene Symbol
DLAT (Dihydrolipoamide S-Acetyltransferase) is a metabolic dehydrogenase involved in adaptive immunity. It is an important research target in fibrotic disease and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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