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KD-Validated Anti-ALKBH1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2365
Application
WB, ICC
Uniprot Id
Q13686
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ALKBH1 antibody (Cat#ABG2365). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALKBH1 antibody (Cat#ABG2365, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ALKBH1 antibody (Cat#ABG2365). ALKBH1 expression in wild type (WT) and ALKBH1 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALKBH1 antibody (Cat#ABG2365, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with ALKBH1 antibody (ABG2365, 1:1,000). Nuclei were stained blue with DAPI; ALKBH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2365-100 3 days 100 µL $499.00
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ABG2365-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ALKBH1 Rabbit Monoclonal Antibody
Accession (Primary)Q13686
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1385
Immunogen
A synthesized peptide derived from ALKBH1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 44 kDa; Observed, 43 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q13686 )

Gene NameALKBH1
Other Gene Names
ALKBH1; AlkB Homolog 1, Histone H2A Dioxygenase; ABH; ALKBH; HABH; Alpha-Ketoglutarate-Dependent Dioxygenase ABH1; Alkylated DNA Repair Protein AlkB Homolog 1; DNA N6-Methyl Adenine Demethylase ALKBH1; MRNA N(3)-Methylcytidine Demethylase; DNA Oxidative Demethylase ALKBH1; Nucleic Acid Dioxygenase ALKBH1; DNA 6mA Demethylase; DNA Lyase ABH1; AlkB; ABH1; AlkB, Alkylation Repair Homolog 1 (E. Coli); AlkB, Alkylation Repair Homolog (E. Coli); TRNA N1-Methyl Adenine Demethylase; AlkB, Alkylation Repair Homolog 1; Alkylation Repair, AlkB Homolog; DNA Demethylase ALKBH1; EC 1.14.11.51; EC 1.14.11.33; EC 1.14.11.-; EC 4.2.99.18; ALKB
About Gene Symbol
ALKBH1 (AlkB Homolog 1, Histone H2A Dioxygenase) is a metabolic enzyme involved in DNA damage response and repair and chromatin remodeling and epigenetics. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and

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Research Use Only
For research use only. Not intended for diagnostic or therapeutic procedures.