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KD-Validated Anti-Bmi1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2369
Application
WB, ICC
Uniprot Id
P35226
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Bmi1 antibody (Cat#ABG2369). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bmi1 antibody (Cat#ABG2369, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Bmi1 antibody (Cat#ABG2369). Bmi1 expression in wild type (WT) and Bmi1 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-Bmi1 antibody (Cat#ABG2369,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Immunocytochemical staining of HeLa cells with Bmi1 antibody (Cat#ABG2369, 1:1,000). Nuclei were stained blue with DAPI; Bmi1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2369-100 3 days 100 µL $499.00
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ABG2369-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Bmi1 Rabbit Monoclonal Antibody
Accession (Primary)P35226
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB685
Immunogen
A synthesized peptide derived from human BMI1
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 37 kDa ; Observed, 40 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P35226 )

Gene NameBMI1
Other Gene Names
BMI1 Proto-Oncogene, Polycomb Ring Finger; RNF51; PCGF4; Polycomb Group RING Finger Protein 4; Polycomb Complex Protein BMI-1; B Lymphoma Mo-MLV Insertion Region 1 Homolog (Mouse); Murine Leukemia Viral (Bmi-1) Oncogene Homolog; B Lymphoma Mo-MLV Insertion Region 1 Homolog; BMI1 Polycomb Ring Finger Proto-Oncogene; BMI1 Polycomb Ring Finger Oncogene; Polycomb Group Ring Finger 4; Polycomb Group Protein Bmi1; Ring Finger Protein 51; RING Finger Protein 51; Flvi-2/Bmi-1; FLVI2/BMI1
About Gene Symbol
BMI1 (BMI1 Proto-Oncogene, Polycomb Ring Finger) is a proto-oncogene involved in gene transcription and regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targe
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