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KD-Validated Anti-Cyclin D1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2370
Application
WB, FC, ICC
Uniprot Id
P24385
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Cyclin D1 antibody (Cat#ABG2370). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin D1 antibody (Cat#ABG2370, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Cyclin D1 antibody (Cat#ABG2370). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin D1 antibody (Cat#ABG2370, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#ABG2370, 1:2,000). Green, isotype control; red, Cyclin D1.
  • Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#ABG2370, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2370-100 3 days 100 µL $499.00
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ABG2370-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin D1 Rabbit Monoclonal Antibody
Accession (Primary)P24385
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB735
Immunogen
A synthesized peptide derived from human Cyclin D1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 34 kDa ; Observed, 34 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:2,000-1:10,000 Immunocytochemical(IC): 1:1,000 Flow cytometry (FCM): 1:2,000

Target Information: ( P24385 )

Gene NameCCND1
Other Gene Names
CCND1; Cyclin D1; U21B31; PRAD1; BCL1; B-Cell Lymphoma 1 Protein; G1/S-Specific Cyclin-D1; B-Cell CLL/Lymphoma 1; BCL-1 Oncogene; PRAD1 Oncogene; D11S287E; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1); Parathyroid Adenomatosis 1; G1/S-Specific Cyclin D1; BCL-1
About Gene Symbol
CCND1 (Cyclin D1) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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