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KD-Validated Anti-Tuberin Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2388
Application
WB, FC, ICC
Uniprot Id
P49815
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Flow cytometric analysis of Tuberin expression in HeLa cells using Tuberin antibody (Cat#ABG2388, 1:2,000). Green, isotype control; red, Tuberin.
  • Immunocytochemical staining of HeLa cells with Tuberin antibody (Cat#ABG2388, 1:1,000). Nuclei were stained blue with DAPI; Tuberin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
  • Western blotting analysis using anti-Tuberin antibody (Cat#ABG2388). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Tuberin antibody (Cat#ABG2388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Tuberin antibody (Cat#ABG2388). Tuberin expression in wild type (WT) and Tuberin shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Tuberin antibody (Cat#ABG2388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2388-100 3 days 100 µL $499.00
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ABG2388-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Tuberin Rabbit Monoclonal Antibody
Accession (Primary)P49815
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1000
Immunogen
A synthesized peptide derived from human tuberin
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 201 kDa ; Observed, 200 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry(FCM):1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P49815 )

Gene NameTSC2
Other Gene Names
Tuberin; PPP1R160; TSC4; LAM; Protein Phosphatase 1, Regulatory Subunit 160; Tuberous Sclerosis 2 Protein; Tuberous Sclerosis 2
About Gene Symbol
TSC2 (Tuberin) is a protein phosphatase involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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