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KD-Validated Anti-Glutathione synthetase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2397
Application
WB, FC, ICC
Uniprot Id
P48637
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Glutathione synthetase antibody (Cat#ABG2397). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutathione synthetase antibody (Cat#ABG2397, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Glutathione Synthetase antibody (Cat#ABG2397). Glutathione Synthetase expression in wild type (WT) and Glutathione Synthetase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Glutathione Synthetase antibody (Cat#ABG2397, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Glutathione synthetase expression in HepG2 cells using Glutathione synthetase antibody (Cat#ABG2397, 1:2,000). Green, isotype control; red, Glutathione synthetase.
  • Immunocytochemical staining of HepG2 cells with Glutathione synthetase antibody (Cat#ABG2397, 1:1,000). Nuclei were stained blue with DAPI; Glutathione synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2397-100 3 days 100 µL $499.00
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ABG2397-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Glutathione synthetase Rabbit Monoclonal Antibody
Accession (Primary)P48637
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4385
Immunogen
A synthesized peptide derived from human Glutathione Synthetase
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 52 kDa; Observed, 48 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P48637 )

Gene NameGSS
Other Gene Names
GSS; Glutathione Synthetase; Glutathione Synthase; GSH Synthetase; EC 6.3.2.3; GSH-S; HEL-S-64p; HEL-S-88n; GSHS
About Gene Symbol
GSS (Glutathione Synthetase) is a biosynthetic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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