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KD-Validated Anti-Cathepsin B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2398
Application
WB, ICC
Uniprot Id
P07858
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Cathepsin B antibody (Cat#ABG2398). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cathepsin B antibody (Cat#ABG2398, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Cathepsin B antibody (Cat#ABG2398). Cathepsin B expression in wild type (WT) and Cathepsin B shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cathepsin B antibody (Cat#ABG2398, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with Cathepsin B antibody (Cat#ABG2398, 1:1,000). Nuclei were stained blue with DAPI; Cathepsin B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2398-100 3 days 100 µL $499.00
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ABG2398-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cathepsin B Rabbit Monoclonal Antibody
Accession (Primary)P07858
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1130
Immunogen
A synthesized peptide derived from human Cathepsin B
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 38 kDa , observed, 38 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P07858 )

Gene NameCTSB
Other Gene Names
CTSB; Cathepsin B; APP Secretase; Cathepsin B1; EC 3.4.22.1; APPS; CPSB; Keratolytic Winter Erythema (Oudtshoorn Skin Disease); Epididymis Secretory Sperm Binding Protein; Amyloid Precursor Protein Secretase; Cysteine Protease; EC 3.4.22; RECEUP; KWE
About Gene Symbol
CTSB (Cathepsin B) is a lysosomal cathepsin protease involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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