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KO-Validated Anti-GAPDH Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG2424
Application
WB, FC
Uniprot Id
P04406
Reactivity
Human, Mouse, Rat
Isotype
Mouse IgG
 
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  • Western blotting analysis using anti-GAPDH antibody (Cat#ABG2424). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GAPDH antibody (Cat#ABG2424, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-GAPDH antibody (Cat#ABG2424). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GAPDH antibody (Cat#ABG2424, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of GAPDH expression in HepG2 cells using anti-GAPDH antibody (Cat#ABG2424, 1:2,000). Green, isotype control; red, GAPDH.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2424-100 3 days 100 µL $499.00
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ABG2424-20 3 days 20 µL $149.00
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Product Overview

NameKO-Validated Anti-GAPDH Mouse Monoclonal Antibody
Accession (Primary)P04406
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG
Clone Names24GB7870
Immunogen
A synthesized peptide derived from human GAPDH
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 36 kDa; observed, 36 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:4,000-1:20,000Flow Cytometry (FCM): 1:2,000

Target Information: ( P04406 )

Gene NameGAPDH
Other Gene Names
GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; GAPD; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein; OCAS, P38 Component; HEL-S-162eP; EC 2.6.99.-; EC 1.2.1; G3PD
About Gene Symbol
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is a metabolic dehydrogenase involved in gene transcription and regulation. It is an important research target in aging research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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