KO-Validated Anti-Protein Regulator Of Cytokinesis 1 Rabbit Monoclonal Antibody

Western blotting analysis using anti-protein regulator of cytokinesis 1 antibody (Cat#ABG2432). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein regulator of cytokinesis 1 antibody (Cat#ABG2432, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-protein regulator of cytokinesis 1 antibody (Cat#ABG2432). Protein regulator of cytokinesis 1 expression in wild-type (WT) and protein regulator of cytokinesis 1 (PRC1) knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein regulator of cytokinesis 1 antibody (Cat#ABG2432, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Protein regulator of cytokinesis 1 expression in HepG2 cells using anti-Protein regulator of cytokinesis 1 antibody (Cat#ABG2432, 1:2,000). Green, isotype control; red, Protein regulator of cytokinesis 1.

Immunocytochemical staining of HepG2 cells with anti-Protein regulator of cytokinesis 1 antibody (Cat#ABG2432, 1:1,000). Nuclei were stained blue with DAPI; Protein regulator of cytokinesis 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.