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KO-Validated Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2433
Application
WB, FC
Uniprot Id
P84022
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-phospho-Smad3 (S423 + S425) antibody (Cat#ABG2433). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Smad3 (S423 + S425) antibody (Cat#ABG2433, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-smad3 (S423 + S425) antibody (Cat#ABG2433). Phospho-smad3 (S423 + S425) expression in wild-type (WT) and SMAD3 knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-smad3 (S423 + S425) antibody (Cat#ABG2433, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Phospho-Smad3 (S423 + S425) expression in HAP-1 cells using anti-Phospho-Smad3 (S423 + S425) antibody (Cat#ABG2433, 1:2,000). Green, isotype control; red, Phospho-Smad3 (S423 + S425).
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2433-100 3 days 100 µL $499.00
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ABG2433-20 3 days 20 µL $149.00
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Product Overview

NameKO-Validated Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody
Accession (Primary)P84022
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3215
Immunogen
A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 61 kDa; observed, 60 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000

Target Information: ( P84022 )

Gene NameSMAD3
Other Gene Names
SMAD3; SMAD Family Member 3; JV15-2; HsT17436; MADH3; Mothers Against Decapentaplegic Homolog 3; Mothers Against DPP Homolog 3; MAD Homolog 3; HMAD-3; HSMAD3; Mad3; MAD, Mothers Against Decapentaplegic Homolog 3 (Drosophila); SMAD, Mothers Against DPP Homolog 3 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 3; SMAD, Mothers Against DPP Homolog 3; SMA- And MAD-Related Protein 3; Mad Protein Homolog; Mad Homolog JV15-2; HSPC193; SMAD 3; LDS1C; Smad3; LDS3
About Gene Symbol
SMAD3 (SMAD Family Member 3) is a transcription factor involved in TGF-β signaling. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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