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KO-Validated Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2436
Application
WB, FC, ICC
Uniprot Id
P46937
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#ABG2436). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#ABG2436, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#ABG2436). Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#ABG2436, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using anti-Phospho-YAP1 (S127) antibody (Cat#ABG2436, 1:2,000). Green, isotype control; red, Phospho-YAP1 (S127).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-YAP1 (S127) antibody (Cat#ABG2436, 1:1,000). Nuclei were stained blue with DAPI; Phospho-YAP1 (S127) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2436-100 3 days 100 µL $499.00
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ABG2436-20 3 days 20 µL $149.00
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Product Overview

NameKO-Validated Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody
Accession (Primary)P46937
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3280
Immunogen
A synthesized peptide derived from human YAP1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 54 kDa; observed, 65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:4,000-1:20,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P46937 )

Gene NameYAP1
Other Gene Names
YAP1; Yes1 Associated Transcriptional Regulator; YAP65; YAP-1; Yes-Associated Protein YAP65 Homolog; Transcriptional Coactivator YAP1; Yes Associated Protein 1; Protein Yorkie Homolog; Yes-Associated Protein 1, 65kDa; 65 KDa Yes-Associated Protein; Yes-Associated Protein 2; Yes-Associated Protein 1; Yorkie Homolog; COB1; YAP2; YAP; YKI
About Gene Symbol
YAP1 (Yes1 Associated Transcriptional Regulator) is a transcriptional coactivator involved in gene transcription and regulation. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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