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KO-Validated Anti-FTO Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG2440
Application
WB, FC
Uniprot Id
Q9C0B1
Reactivity
Human, Mouse, Rat
Isotype
Mouse IgG1
 
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  • Western blotting analysis using anti-FTO antibody (Cat#ABG2440). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FTO antibody (Cat#ABG2440, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-FTO antibody (Cat#ABG2440). FTO expression in wild-type (WT) and FTO knockout (KO) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FTO antibody (Cat#ABG2440, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of FTO expression in HeLa cells using anti-FTO antibody (Cat#ABG2440, 1:2,000). Green, isotype control; red, FTO.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2440-100 3 days 100 µL $499.00
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ABG2440-20 3 days 20 µL $149.00
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Product Overview

NameKO-Validated Anti-FTO Mouse Monoclonal Antibody
Accession (Primary)Q9C0B1
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone Names25GB3740
Immunogen
Recombinant protein of human FTO
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 58 kDa; observed, 58 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:500-1:2,500Flow Cytometry (FCM): 1:2,000

Target Information: ( Q9C0B1 )

Gene NameFTO
Other Gene Names
FTO; FTO Alpha-Ketoglutarate Dependent Dioxygenase; KIAA1752; ALKBH9; U6 Small Nuclear RNA (2'-O-Methyladenosine-N(6)-)-Demethylase FTO; U6 Small Nuclear RNA N(6)-Methyladenosine-Demethylase FTO; MRNA (2'-O-Methyladenosine-N(6)-)-Demethylase FTO; Alpha-Ketoglutarate-Dependent Dioxygenase FTO; Intragenic FTO Exon 9 Containing Transcript; MRNA N(6)-Methyladenosine Demethylase FTO; Fat Mass And Obesity-Associated Protein; TRNA N1-Methyl Adenine Demethylase FTO; Fat Mass And Obesity Associated; M6A(M)-Demethylase FTO; AlkB Homolog 9; MGC5149; IFEX9; FTO, Alpha-Ketoglutarate; Dependent Dioxygenase; Alpha-Ketoglutarate-Dependent Dioxygenase; EC 1.14.11.53; EC 1.14.11.-; BMIQ14; GDFD
About Gene Symbol
FTO (FTO Alpha-Ketoglutarate Dependent Dioxygenase) is a metabolic enzyme involved in translational regulation. It is an important research target in metabolic disease and is widely studied to elucidate cellular mechanisms and identify therapeutic targets
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