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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| ABM0256-500 | 7 days | 500 µg | $550.00 |
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| ABM0256-1 | 7 days | 1 mg | $880.00 |
Product Overview | |
| Name | FLAG Antibody |
|---|---|
| Description | |
| FLAG peptide, Mouse Antibody | |
| Introduction | |
| A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. | |
| Formulation | |
| 1×PBS & 50% glycerol. | |
| Applications | |
| Western Blot, Immunoprecipitation. | |
| Type | |
| Mouse Antibody Monoclonal. | |
| Clone | |
| PFLAGSHG. | |
| Ig Subclass | |
| Mouse IgG2b. | |
| Titer | |
| Western Blotting: 0.5 µg/ml. | |
| Precautions | |
| FLAG Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
ADMEbio welcomes feedback from our customers.
Thank you,
ADMEbio Team
