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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| ABM0441-5 | 7 days | 5 µg | $120.00 |
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| ABM0441-20 | 7 days | 20 µg | $290.00 | |||
| ABM0441-100 | 7 days | 100 µg | $720.00 |
Product Overview | |
| Name | Leptin PAT7E10AT Antibody |
|---|---|
| Description | |
| Leptin Clone PAT7E10AT, Mouse Anti Human | |
| Synonyms | |
| OB Protein, Obesity Protein, OBS, Obesity factor. | |
| Introduction | |
| A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO 3 . | |
| Stability | |
| Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Trp. | |
| Biological Activity | |
| Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized recombinant porcine leptin in sterile 0.4% NaHCO 3 adjusted to ph 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Protein content | |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Porcine as a Reference Standard. | |
| Precautions | |
| Leptin PAT7E10AT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
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Thank you,
ADMEbio Team
