Hello, Sign in
My Account
My Account
New customer? Start here
| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| ABM0466-500 | 7 days | 500 µg | $550.00 |
Select product before adding to cart
|
||
| ABM0466-1 | 7 days | 1 mg | $880.00 |
Product Overview | |
| Name | MHC Class I, H-2K Antibody |
|---|---|
| Description | |
| MHC Class I, (H-2K) Mouse Antibody | |
| Immunogen | |
| Purified mouse LN cells (C57Bl/6 anti-BALB/c). | |
| Introduction | |
| MHC Class I is a membrane spanning molecule consisting of 2 proteins. MHC Class I is just about 350 amino acids in length, with roughly 75 amino acids at the carboxylic end comprising the transmembrane and cytoplasmic portions. The remaining 270 amino acids, are divided into three globular domains labeled Alpha-1, Alpha-2 and Alpha-3 prime, with Alpha-1 being closest to the amino terminus and Alpha-3 closest to the membrane. The second portion of the molecule is a small globular protein called Beta-2 Microglobulin. It links primarily with the Alpha-3 prime domain and is essential for MHC stability. The MHC ability to show a vast range of antigenic peptides for T cell recognition needs a compromise between broad specificity and high affinity. Both MHC class I and MHC class II show an exceptional structure while the peptide main chain is tightly bound and peptide side chains show less restrictive interactions. It is primarily the peptide side-chain contacts and conformational variability that ensures that the peptide-MHC complex presents an antigenically unique surface to T cell receptors. | |
| Formulation | |
| 1mg/ml in PBS (after reconstitution). | |
| Solubility | |
| Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. | |
| Applications | |
| Cytotoxic and staining antibody. For staining, use 10ml/1,000,000 cells. Titer for cytotoxicity should be determined by the investigator. | |
| Type | |
| Mouse Antibody Monoclonal. | |
| Clone | |
| NYRmH-2K. | |
| Ig Subclass | |
| Mouse IgG2b. | |
| Purification Method | |
| Ion exchange column. | |
| Storage Procedures | |
| Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. | |
| Available Conjugates | |
| This antibody is also available as unconjugated antibody and conjugated to FITC. | |
| Specificity | |
| Recognizes most mouse H-2K haplotypes (k,p,q,r,s). Does NOT react with H-2kd. | |
| Precautions | |
| MHC Class I, H-2K Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
ADMEbio welcomes feedback from our customers.
Thank you,
ADMEbio Team
