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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| ABP0030-100 | 7 days | 100 µg | $330.00 |
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| ABP0030-500 | 7 days | 500 µg | $1,320.00 | |||
| ABP0030-1 | 7 days | 1 mg | $1,920.00 |
Product Overview | |
| Name | T.Vaginalis P65 Antibody |
|---|---|
| Description | |
| Polyclonal Rabbit Anti Trichomonas Vaginalis P65 Antibody | |
| Synonyms | |
| DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4. | |
| Source | |
| Escherichia Colilambda lysogen NM 989. | |
| Physical Appearance | |
| Sterile filtered liquid formulation having a concentration of 167,000 U/ml. | |
| Formulation | |
| 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 µm in 5´ termini). Optimal ligation occurs at 16C. | |
| Biological Activity | |
| One Weiss unit is equivalent to circa 67 cohesive-end ligation units. • T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM. • Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50 µM. • To dilute T4 DNA Ligase that will subsequently be stored at –20°C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used. | |
| Applications | |
| Cloning of restriction fragments. Joining linkers and adapters to blunt-ended DNA. | |
| Unit Definition | |
| 1. One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 ul in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer. 2.One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmol of 32P from pyrophosphate to ATP, into Norit-adsorbable material in 20 minutes at 37°C. | |
| Note | |
| Purified free of contaminating endonucleases and exonucleases. Each lot of T4 DNA ligase is also tested in a mock cloning assay, which reveals any damage to the ligated DNA termini. Greater than 99.9% of the termini remain undamaged in this assay. | |
| Endonuclease Activity | |
| Incubation of a 50 µl reaction containing 13,000 units of T4 DNA Ligase with 1 µg of X174 RF I DNA for 4 hours at 37°C resulted in < 5% conversion to RFII as determined by agarose gel electrophoresis. | |
| Inactivation | |
| T4 DNA Ligase can be inactivated by incubation at 65°C for 10 minutes. | |
| Storage Buffer | |
| 50mM KCl, 10mM Tris-HCl (pH 7.4), 0.1mM EDTA, 1mM DTT, 200 µg/ml BSA and 50% glycerol. Store at -20C. | |
| Exonuclease Activity | |
| Incubation of a 50 µl reaction containing 13,000 units of T4 DNA Ligase with 1 µg of a mixture of single and double-stranded [3H] E. coli DNA (200,000 cpm/ug) for 4 hours at 37°C released < 0.3% of the total radioactivity. | |
| Nuclease Activity | |
| Incubation of 13,000 units for 18 hours in assay buffer (without ATP) with Hind III fragments of gamma DNA yielded a clear and sharp banding pattern on agarose gels. | |
| Precautions | |
| T.Vaginalis P65 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
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Thank you,
ADMEbio Team
