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Raf1 Rabbit pAb

 
Catalog #
ABZ1426
Application
WB, IF, ICC, ELISA, IHC-P
Uniprot Id
P04049
Reactivity
Human, Mouse, Rat
Isotype
IgG
 
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  • Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.
  • Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody(red),and DAPI (blue).
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABZ1426-100 2 weeks 100 µL $499.00
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ABZ1426-20 2 weeks 20 µL $149.00
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Product Overview

NameRaf1 Rabbit pAb
Accession (Primary)P04049
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Calculated MW: 73 kDa; Observed MW: 73 kDa
Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:2000; IHC-1:100-1:300; IF-1:50-1:200; ELISA-1:5000
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Target Information: ( P04049 )

NameRAF1
Gene ID5894
Other Names
RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
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