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>   Home   >   Products   >   Primary Antibodies   >   POLR2A Rabbit mAb   

POLR2A Rabbit mAb

 
Catalog #
ABZ3247
Application
WB, IF, FC, IP, ICC, IHC-P
Uniprot Id
P24928
Reactivity
Human, Mouse, Rat
Isotype
IgG
 
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  • Immunocytochemistry analysis of RNA Polymerase II Subunit B1 (green) in CEM using RNA Polymerase II Subunit B1 antibody,and DAPI(blue).
  • Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using RNA polymerase II CTD repeat YSPTSPS antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABZ3247-100 2 weeks 100 µL $499.00
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ABZ3247-20 2 weeks 20 µL $149.00
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Product Overview

NamePOLR2A Rabbit mAb
Accession (Primary)P24928
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeIgG
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Calculated MW: 217 kDa; Observed MW: 250 kDa
Buffer System
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:1000; IHC-P-1:200-1:1000; ICC/IF-1:200-1:500; FC-1:50-1:100; IP-1:20-1:50
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Target Information: ( P24928 )

NamePOLR2A
Gene ID5430
Other Names
RNA Polymerase II Subunit B1; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
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