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[KD]p150 CAF1 Rabbit mAb

Catalog #: ABZ4657
Application: WB, IF, FC, ICC, IHC-P
Uniprot Id: Q13111
Reactivity: Human
Isotype: IgG

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Western blot analysis of p150 CAF1 in HeLa(WT) and p150 CAF1-Knockdown HeLa(KD) using p150 CAF1 antibody.
Western blot analysis of p150 CAF1 in K562 lysates using p150 CAF1 antibody.
Immunocytochemistry analysis of p150 CAF1(green) in Hela using p150 CAF1 antibody,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using p150 CAF1/CAF antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	[KD]p150 CAF1 Rabbit mAb
Accession (Primary)	Q13111
Host Species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Reacts With	Human
Calculated MW
Calculated MW: 107 kDa; Observed MW: 150 kDa
Buffer System
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:1000-1:5000; IHC-P-1:100-1:200; ICC/IF-1:20-1:50; FC-1:20-1:50
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Target Information: ( Q13111 )
Name	CHAF1A
Gene ID	10036
Other Names
CHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150

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