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[KO]PRAME Rabbit mAb

Catalog #: ABZ4665
Application: WB, IF, FC, IP, ICC, IHC-P
Uniprot Id: P78395
Reactivity: Human
Isotype: IgG

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Western blot analysis of PRAME in HCT116(WT) and PRAME-Knockout HCT116(KO) lysates using PRAME antibody.
Immunohistochemistry analysis of paraffin-embedded mouse testis using PRAME antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Western blot analysis of PRAME in K562 lysates using PRAME antibody.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	[KO]PRAME Rabbit mAb
Accession (Primary)	P78395
Host Species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Reacts With	Human
Calculated MW
Calculated MW: 58 kDa; Observed MW: 58 kDa
Buffer System
1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:1000-1:10000; IHC-1:100-1:500; ICC/IF-1:200-1:500; FC-1:200-1:500; IP-1:20-1:50
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980).
Target Information: ( P78395 )
Name	PRAME
Gene ID	23532
Other Names
PRAME; MAPE; OIP4; Opa-interacting protein 4(OIP-4); Preferentially expressed antigen of melanoma

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