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[KD]MCM5 Rabbit mAb

Catalog #: ABZ4687
Application: WB, IF, ICC, IHC-P
Uniprot Id: P33992
Reactivity: Human
Isotype: IgG

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Western blot analysis of MCM5 in HeLa(WT) and MCM5-Knockdown HeLa(KD) using MCM5 antibody.
Western blot analysis of MCM5 in Jurkat, C6, Hela lysates using MCM5 antibody.
Immunocytochemistry analysis of MCM5(green) in Hela using MCM5 antibody,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM5 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	[KD]MCM5 Rabbit mAb
Accession (Primary)	P33992
Host Species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Reacts With	Human
Calculated MW
Calculated MW: 82 kDa; Observed MW: 82 kDa
Buffer System
1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:2000; IHC-1:100-1:2000; IF-1:50-1:200
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.
Target Information: ( P33992 )
Name	MCM5
Gene ID	4174
Other Names
CDC46; MCM5; MGC5315; P1 CDC46

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