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[KO]Cofilin 1 Rabbit mAb

Catalog #: ABZ4736
Application: WB, IF, ICC, IHC-P
Uniprot Id: P23528
Reactivity: Human, Mouse, Rat
Isotype: IgG

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Western blot analysis of Cofilin in HeLa(WT) and Cofilin-Knockout HeLa(KO) using Cofilin antibody.
Western blot analysis of Cofilin in A549, MCF-7, 293T lysates using Cofilin antibody.
Immunocytochemistry analysis of Cofilin (green) in hela using Cofilin antibody,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cofilin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	[KO]Cofilin 1 Rabbit mAb
Accession (Primary)	P23528
Host Species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Reacts With	Human, Mouse, Rat
Calculated MW
Calculated MW: 19 kDa; Observed MW: 19 kDa
Buffer System
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:1000-1:5000; IHC-P-1:100-1:200; IF-1:50-1:200
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Target Information: ( P23528 )
Name	CFL1
Gene ID	1072
Other Names
CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform

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