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[KO]PSMB8 (3G3) Mouse mAb

Catalog #: ABZ4808
Application: WB, IF, ICC, IHC-F, IHC-P
Uniprot Id: P28062
Reactivity: Human, Rat
Isotype: IgG1

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Western blot analysis of PSMB8/LMP7 in C6, Hela, Molt4 and Raji lysates using PSMB8/LMP7 antibody.
Immunofluorescence analysis of Proteasome beta 8 (3G3) in Hela cells using PSMB8 antibody(green),and DAPI (blue)
Immunohistochemistry analysis of paraffin-embedded intima cancer tissues using PSMB8 antibody with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded colon tissues using Proteasome beta 8 (3G3) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	[KO]PSMB8 (3G3) Mouse mAb
Accession (Primary)	P28062
Host Species	Mouse
Clonality	Monoclonal
Isotype	IgG1
Reacts With	Human, Rat
Calculated MW
Calculated MW: 30 kDa; Observed MW: 23,30 kDa
Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:1000; IHC-1:50-1:100; IF-1:50-1:200
Purification
Ascitic Fluid
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Target Information: ( P28062 )
Name	PSMB8
Gene ID	5696
Other Names
JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E; Proteasome 20S LMP7/PSMB8

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