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Alkaline Phosphatase Rabbit mAb

 
Catalog #
ABZ5248
Application
WB, IF, ICC, IHC-P
Uniprot Id
P05186
Reactivity
Human, Mouse, Rat
Isotype
IgG
 
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  • Western blot analysis of Alkaline Phosphatase in (1)HepG2 lysates; (2)JAR lysates using Alkaline Phosphatase antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human liver using Alkaline Phosphatase antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunofluorescence analysis of Alkaline Phosphatase in Hela using Alkaline Phosphatase antibody.
  • Western blot analysis of Alkaline Phosphatase in SKOV-3, Jurkat and MCF-7 lysates using Alkaline Phosphatase antibody.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABZ5248-100 2 weeks 100 µL $499.00
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ABZ5248-20 2 weeks 20 µL $149.00
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Product Overview

NameAlkaline Phosphatase Rabbit mAb
Accession (Primary)P05186
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeIgG
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Calculated MW: 57 kDa; Observed MW: 75 kDa
Buffer System
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:1000; IHC-1:50-1:100; IF-1:50-1:200; IP-1:20; FC-1:50-1:100
Purification
Affinity Chromatography
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Target Information: ( P05186 )

NameALPL
Gene ID249
Other Names
ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
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