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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List |
|---|
Product Overview | |
| Name | Avidin Biotin |
|---|---|
| Description | |
| Modified Affinity Purified Avidin | |
| Accession (Primary) | P02701 |
| Synonyms | |
| Thrombin-like enzyme batroxobin, EC 3.4.21.74, BX, Bothrops atrox serine proteinase, Venombin-A, Defibrase, Reptilase, Batroxobin. | |
| Introduction | |
| Batroxobin is a serin protease that reduces fibronogen levels and is originally extracted from snake venom of Bothrops Atrox. Batroxobin is used in defibrinogenation and thrombolysis and also has an effect on c-fos gene and growth factor. Batroxobin can efficiently restrain proliferation of VSMCs, by blocking the release and uptake of Ca2+, thus influencing [Ca2+]i. Batroxobin is a single chain glycopeptide with a molecular mass of 43kDa on SDS-PAGE gel and its pI-6.6. Batroxobin converts fibrinogen to fibrin through the restricted release of fibrinopeptide-A from fibrinogen to promote blood to clot. Unlike thrombin, it is not affected by heparin and hirudin. | |
| Source | |
| Pichia Pastoris. | |
| Physical Appearance | |
| Sterile Filtered white lyophilized powder. | |
| Formulation | |
| The Batroxobin protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM sodium acetate buffer, pH 7.4. | |
| Stability | |
| Batroxobin although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution Batroxobin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P. | |
| Biological Activity | |
| Batroxobin’s biological activity was found to be of no less than 500KU/mg. (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37°C in vitro within 60 ± 20 seconds. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Batroxobin in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| Title :Impaired clot retraction in factor XIII A subunit-deficient mice Supplemental materials for: Kasahara et al Publication :Published online before print December 8, 2009, doi: 10.1182/blood-2009-06-227645 Blood December 8, 2009 Link : http://bloodjournal.hematologylibrary.org/content/115/6/1277/suppl/DC1 | |
| Precautions | |
| Avidin Biotin is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P02701 ) | |
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