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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CDA0149-2 | 7 days | 2 µg | $120.00 |
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| CDA0149-10 | 7 days | 10 µg | $290.00 | |||
| CDA0149-100 | 7 days | 100 µg | $1,920.00 |
Product Overview | |
| Name | sCD40L Human, His Active |
|---|---|
| Description | |
| soluble CD40 Ligand/TRAP His Tag Active Human Recombinant | |
| Accession (Primary) | P29965 |
| Synonyms | |
| CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L. | |
| Introduction | |
| CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines . CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma , TNF-alpha , and IL2 . | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a sterile concentrated solution (1mg/ml) with 10mM Sodium Phosphate pH=7.5. | |
| Stability | |
| Lyophilized sCD40 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD154 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Arg-Gly-Asp. | |
| Biological Activity | |
| The ED50 as determined by its ability to induce MIP-1 alpha & TNF-alpha from mouse splenocytes was found to be 0.1 µg/ml corresponding to a Specific Activity of 10,000IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized sCD40 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Precautions | |
| sCD40L Human, His Active is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P29965 ) | |
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ADMEbio Team
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ADMEbio Team
