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CD4 Human (26-226)

CD-4 (26-226 a.a.) Human Recombinant

 
Catalog #
CDA0153
 
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Product Overview

NameCD4 Human (26-226)
Description
CD-4 (26-226 a.a.) Human Recombinant
Immunogen
Anti-human CD45 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD45 amino acids 1029-1249 purified from E. coli.
Synonyms
Leukocyte common antigen, EC 3.1.3.48, L-CA, T200, CD45 antigen, PTPRC, LCA, LY5, B220, CD45, GP180.
Introduction
CD45 leukocyte common antigen (LCA) belongs to the family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of these molecules has protein phosphatase activity and is involved in regulation of transmembrane signals.
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Applications
CD45 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
Type
Mouse Anti Human Monoclonal.
Clone
P3G4AT.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.
Purification Method
CD45 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Precautions
CD4 Human (26-226) is for research use only and not for use in diagnostic or therapeutic procedures.
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