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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CHK0125-5 | 7 days | 5 µg | $120.00 |
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| CHK0125-20 | 7 days | 20 µg | $290.00 | |||
| CHK0125-1 | 7 days | 1 mg | $4,320.00 |
Product Overview | |
| Name | MIP 1a Rat |
|---|---|
| Description | |
| Macrophage Inflammatory Protein-1 Alpha Rat Recombinant (CCL3) | |
| Accession (Primary) | P50229 |
| Synonyms | |
| Small inducible cytokine A4, CCL4, Macrophage inflammatory protein 1-beta, MIP-1- beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, H400, SIS-gamma, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, chemokine (C-C motif) ligand 4, ACT2, G-26, LAG1, MIP1B, SCYA4, AT744.1, MGC104418, MGC126025, MGC126026. | |
| Introduction | |
| Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. | |
| Stability | |
| Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser. | |
| Biological Activity | |
| The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| Title : CONFUSION BETWEEN CCL4 AND CCL4L1: AN EXAMPLE TO BEAR IN MIND WHEN USING THIRD-PARTY REAGENTS. Publication : Inmunologia Vol. 26 / Num 1/ Enero-Marzo 2007: 51-54 (Spanish). Link: MIP 1b prospec publication | |
| Precautions | |
| MIP 1a Rat is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P50229 ) | |
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