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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0314-2 | 7 days | 2 µg | $120.00 |
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| CYK0314-10 | 7 days | 10 µg | $290.00 | |||
| CYK0314-1 | 7 days | 1 mg | $4,320.00 |
Product Overview | |
| Name | IL 3 Dog |
|---|---|
| Description | |
| Interleukin-3 Canine Recombinant | |
| Accession (Primary) | Q9BDX4 |
| Synonyms | |
| MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. | |
| Introduction | |
| IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3. | |
| Stability | |
| Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln. | |
| Biological Activity | |
| The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| 1. Title :Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice. Publication :Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 . Link : IL 3 prospec publication 2. Title :A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single-Cell Dissociation. Publication :Article first published online: 24 AUG 2006 DOI:10.1634/stemcells.2005-0657 Copyright © 2006 AlphaMed Press Link : IL 3 Human prospec publication 3. Title: Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent Pathway Publications: PloS one 8.12 (2013): e82867 Link: Interleukin-3 prospec publication 4. Title: Novel markers of normal and neoplastic human plasmacytoid dendritic cells Publication: Blood 111.7 (2008): 3778-3792. Link: Interleukin-3 Human prospec publication | |
| Protein content | |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard. | |
| Precautions | |
| IL 3 Dog is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( Q9BDX4 ) | |
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ADMEbio Team
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