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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0317-2 | 7 days | 2 µg | $120.00 |
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| CYK0317-10 | 7 days | 10 µg | $290.00 | |||
| CYK0317-1 | 7 days | 1 mg | $5,760.00 |
Product Overview | |
| Name | IL 3 Human, Sf9 |
|---|---|
| Description | |
| Interleukin-3 Human Recombinant, Sf9 | |
| Accession (Primary) | P08700 |
| Synonyms | |
| MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. | |
| Introduction | |
| Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. | |
| Stability | |
| Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 97.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg. | |
| Biological Activity | |
| The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| 1. Title : Direct binding of Cbl to Y568 and Y936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation. Publication :Biochemical Journal Immediate Publication. Published on 19 Jun 2006 as manuscript BJ20060464. Link : IL 3 Mouse prospec publication 2. Title :enhanced self -renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene sall4. Publication : Link : Interleukin-3 Mouse prospec publication 3 . Title :Identification of Y589 and Y599 in the juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2. Publication :Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896 Submitted: July 14, 2005Accepted: April 26, 2006 Blood September 1, 2006vol. 108no. 51542-1550. Link : http://bloodjournal.hematologylibrary.org/content/108 /5/1542.full | |
| Protein content | |
| Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL3 as a Reference Standard. | |
| Precautions | |
| IL 3 Human, Sf9 is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P08700 ) | |
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