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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0348-2 | 7 days | 2 µg | $120.00 |
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| CYK0348-10 | 7 days | 10 µg | $290.00 | |||
| CYK0348-1 | 7 days | 1 mg | $5,440.00 |
Product Overview | |
| Name | IL 7 Human, His |
|---|---|
| Description | |
| Interleukin-7 Human Recombinant, His Tag | |
| Accession (Primary) | P13232 |
| Synonyms | |
| Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7. | |
| Introduction | |
| IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. | |
| Source | |
| Saccharomyces cerevisiae. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer. | |
| Stability | |
| Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu. | |
| Biological Activity | |
| The ED 50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 10 6 units/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Protein content | |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard. | |
| Precautions | |
| IL 7 Human, His is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P13232 ) | |
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ADMEbio Team
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