| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0349-2 | 7 days | 2 µg | $120.00 |
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| CYK0349-10 | 7 days | 10 µg | $290.00 | |||
| CYK0349-1 | 7 days | 1 mg | $7,488.00 |
Product Overview | |
| Name | IL 7 Human, Yeast |
|---|---|
| Description | |
| Interleukin-7 Human Recombinant, Yeast | |
| Accession (Primary) | P13232 |
| Synonyms | |
| Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7. | |
| Introduction | |
| Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Filtered (0.2 µm) and lyophilized from a concentrated (1mg/ml) solution in 1×PBS, pH7.4 and 2% trehalose. | |
| Stability | |
| Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI. | |
| Biological Activity | |
| The ED 50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0×10 6 IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| Title: Disruption of Bis Leads to the Deterioration of the Vascular Niche for Hematopoietic Stem Cells Publication: Stem Cells 28.2 (2010): 268-278. Link: IL 7 Mouse prospec publication | |
| Precautions | |
| IL 7 Human, Yeast is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P13232 ) | |
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