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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0356-2 | 7 days | 2 µg | $120.00 |
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| CYK0356-10 | 7 days | 10 µg | $290.00 | |||
| CYK0356-1 | 7 days | 1 mg | $8,320.00 |
Product Overview | |
| Name | IL-1 Alpha Rat, His Active |
|---|---|
| Description | |
| Interleukin-1 alpha Rat Recombinant, His Tag Active | |
| Accession (Primary) | P16598 |
| Synonyms | |
| B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. | |
| Introduction | |
| Recombinant IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a 0.2 µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. | |
| Stability | |
| Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N. | |
| Biological Activity | |
| The ED 50 as determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells was found to be less than 2.0ng/ml, corresponding to a specific activity of 5.0×10 5 IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| 1. Title :Activated signal transducer and activator of transcription-3 (STAT3) is a poor regulator of tumour necrosis factor- production by human monocytes. Publication :Article first published online: 9 JAN 2007 DOI:10.1111/j.1365-2249.2006.03291.x Link : IL10 prospec publication 2. Title :Suppressor of cytokine signalling-3 at pathological levels does not regulate lipopolysaccharide or interleukin-10 control of tumour necrosis factor-? production by human monocytes. Publication :Article first published online: 11 MAY 2006 DOI: 10.1111/j.1365-2567.2006.02383.x Link : IL10 Human prospec publication 3. Title :The Tyrosine Sulfate-rich Domains of the LRR Proteins Fibromodulin and Osteoadherin Bind Motifs of Basic Clusters in a Variety of HPR-binding Proteins, Including Bioactive Factors*. Publication :First Published on August 21, 2009, doi: 10.1074/jbc.M109.047076 October 16, 2009 The Journal of Biological Chemistry, 284, 28543-28553. Link : Interleukin-10 prospec publication | |
| Precautions | |
| IL-1 Alpha Rat, His Active is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P16598 ) | |
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