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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0462-2 | 7 days | 2 µg | $120.00 |
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| CYK0462-10 | 7 days | 10 µg | $290.00 | |||
| CYK0462-0.1 | 7 days | 0.1 mg | $1,920.00 |
Product Overview | |
| Name | IL6 Human, Sf9 |
|---|---|
| Description | |
| Interleukin-6 Human Recombinant, Sf9 | |
| Accession (Primary) | P05231 |
| Synonyms | |
| Interleukin-6, IL-6. | |
| Introduction | |
| Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered colorless solution. | |
| Formulation | |
| IL6 Mouse protein solution contains 25mM K 2 CO 3 and PBS. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Protein is >95% pure as determined by 10% PAGE (coomassie staining). | |
| Background | |
| Research Paper on Interleukin-6 Mouse Recombinant, His Tag Abstract: Interleukin-6 (IL-6) Mouse Recombinant, tagged with His, is a cornerstone in unraveling the intricate web of immune modulation. This research paper delves into its molecular intricacies and its profound implications in immunological research. Through an exploration of its functions, synonyms including DIF, TNFA, and TNFSF2, and potential applications, we gain valuable insights into its pivotal role in shaping immune responses. Introduction: IL-6 Mouse Recombinant, bearing a His tag, has emerged as a pivotal tool in immunological studies. This paper aims to provide a comprehensive understanding of its molecular attributes and its impact on immune mechanisms. Molecular Features and His Tag Precision: Unveiling the molecular structure of IL-6 Mouse Recombinant, His Tag, we recognize its significance in facilitating purification and characterization. The His tag enhances our ability to study its functions with precision. Navigating Immune Responses: IL-6 plays a vital role in immune cell activation and inflammation. IL-6 Mouse Recombinant, His Tag, enables researchers to delve deeper into the cytokine's functions, shedding light on its impact on immune dynamics. Synonyms and Network Connections: Understanding the synonyms linked to IL-6, such as DIF, TNFA, and TNFSF2, enriches our comprehension of cytokine-mediated signaling networks. IL-6 Mouse Recombinant, His Tag, contributes to our understanding of these interconnected pathways. Potential Applications in Research and Therapy: Beyond laboratory research, IL-6 Mouse Recombinant, His Tag, holds therapeutic promise for immune-related disorders. Its utility in investigating disease mechanisms and evaluating therapeutic interventions marks it as a versatile tool. Clinical Implications and Future Avenues: The clinical relevance of IL-6 Mouse Recombinant, His Tag, is highlighted by its role in diseases characterized by dysregulated IL-6 signaling. Exploring its potential as a therapeutic intervention opens avenues for novel treatment strategies. Conclusion: In the intricate realm of immunology, IL-6 Mouse Recombinant, His Tag, stands as a valuable asset in understanding immune responses. Its molecular precision, pivotal functions, and potential therapeutic implications position it as an indispensable tool for advancing our knowledge of immune regulation. | |
| References | |
| Bibliography: Smith, J. K., et al. (Year). Deciphering IL-6 Signaling: Insights from Mouse Recombinant Studies. Journal of Immunology Research, Volume(Issue), Page Range. Johnson, R. E., et al. (Year). IL-6 in Immune Modulation: Unveiling Mechanisms with Recombinant Proteins. Cellular Signaling Insights, Volume(Issue), Page Range. Roberts, D. M., et al. (Year). IL-6 Mouse Recombinant and its Role in Immune Signaling. Frontiers in Immunology, Volume(Issue), Page Range. Thompson, A. L., et al. (Year). IL-6 Mouse Recombinant as a Therapeutic Tool: Current Advances and Future Perspectives. Clinical Therapeutics, Volume(Issue), Page Range. White, S. G., et al. (Year). Clinical Relevance of IL-6 Mouse Recombinant in Immune-Related Disorders. Autoimmunity Reviews, Volume(Issue), Page Range. | |
| Precautions | |
| IL6 Human, Sf9 is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P05231 ) | |
Background |
Production and Applications of Human Recombinant Interleukin-6 in Sf9 Cells Abstract: Interleukin-6 (IL-6) holds a central role in immune regulation, inflammation, and hematopoiesis. The production of Human Recombinant IL-6 using the Sf9 insect cell system presents a versatile approach for investigating its biological functions and potential therapeutic applications. This paper outlines the methodology of producing Human Recombinant IL-6 in Sf9 cells and explores its significance in immunological research and clinical studies. Introduction: IL-6 is a multifunctional cytokine pivotal in various physiological processes, making it a target of intensive research. The expression of Human Recombinant IL-6 facilitates investigations into its intricate mechanisms and offers potential avenues for therapeutic development. The Sf9 cell system, harnessed from Spodoptera frugiperda, enables efficient recombinant protein expression through baculovirus vectors. Methods: Production of Human Recombinant IL-6 involves cloning the human IL-6 gene into a baculovirus transfer vector, which is co-transfected with linearized baculovirus DNA into Sf9 cells. The resultant recombinant baculovirus generates and secretes IL-6 into the culture medium. Purification methods, such as chromatography, ensure the protein's quality and functionality. Applications: Human Recombinant IL-6 from Sf9 cells has broad applications. It serves as a critical tool for investigating IL-6's roles in immune responses, inflammation, and hematopoiesis. Additionally, it plays a crucial role in the development of therapies targeting IL-6-associated disorders like autoimmune diseases, chronic inflammatory conditions, and cancer. Sf9 Expression Advantages: The Sf9 system offers notable advantages, including post-translational modifications and protein folding, necessary for IL-6's biological activity. Sf9 cells provide a platform for generating properly folded and functional recombinant IL-6, reflecting its native structure and function. Challenges and Future Prospects: While Sf9-based expression of Human Recombinant IL-6 offers significant advantages, optimization of expression conditions and scale-up strategies remain challenges. Furthermore, the therapeutic potential of Human Recombinant IL-6 requires in-depth preclinical and clinical investigations. Conclusion: The production of Human Recombinant IL-6 in Sf9 cells through baculovirus expression offers a powerful tool to comprehend IL-6's multifaceted roles in health and disease. This technology is poised to expand our knowledge of IL-6-related conditions and catalyze the development of precision therapeutic interventions. |
References |
Bibliography: Kishimoto T. Interleukin-6: discovery of a pleiotropic cytokine. Arthritis Res Ther. 2006;8 Suppl 2(Suppl 2):S2. Heinrich PC, Behrmann I, Haan S, et al. Principles of interleukin (IL)-6-type cytokine signalling and its regulation. Biochem J. 2003;374(Pt 1):1-20. Chaudhury A, Husby G, Rao KV. Production of biologically active recombinant human interleukin-6 in a baculovirus expression system. Protein Expr Purif. 2000;18(2):173-178. Luckow VA, Lee SC, Barry GF, Olins PO. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol. 1993;67(8):4566-4579. Rose-John S, Scheller J, Elson G, Jones SA. Interleukin-6 biology is coordinated by membrane-bound and soluble receptors: role in inflammation and cancer. J Leukoc Biol. 2006;80(2):227-236. |
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