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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| CYK0479-5 | 7 days | 5 µg | $120.00 |
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| CYK0479-25 | 7 days | 25 µg | $290.00 | |||
| CYK0479-1 | 7 days | 1 mg | $4,032.00 |
Product Overview | |
| Name | LIF Mouse |
|---|---|
| Description | |
| Leukemia Inhibitory Factor Mouse Recombinant | |
| Accession (Primary) | P09056 |
| Synonyms | |
| Leukemia inhibitory factor, Cholinergic neuronal differentiation factor, Lif. | |
| Introduction | |
| Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| LIF Rat was lyophilized from 0.2 µm filtered concentrated solution in 1xPBS, pH 7.4. | |
| Stability | |
| Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF . | |
| Biological Activity | |
| The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5ng/mL. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Precautions | |
| LIF Mouse is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P09056 ) | |
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ADMEbio Team
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ADMEbio Team
