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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List |
|---|
Product Overview | |
| Name | IL 3 Human, HEK |
|---|---|
| Description | |
| Interleukin-3 Human Recombinant, HEK | |
| Accession (Primary) | P08700 |
| Synonyms | |
| BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. | |
| Introduction | |
| IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. | |
| Source | |
| Chinese Hamster Ovarian Cells. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from a Phosphate- Buffered Saline (pH 7.4). | |
| Stability | |
| Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Biological Activity | |
| Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2ng/ml corresponding to a Specific Activity of 5,000,000-20,000,000IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| Title :A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic Disease Publication :doi: 10.4049/jimmunol.0804125 The Journal of Immunology May 15, 2009 vol. 182 no. 10 6011-6021 Link : http://www.jimmunol.org/content/182/10/6011.full | |
| Precautions | |
| IL 3 Human, HEK is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P08700 ) | |
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