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DiMethyl-Histone H3 (Lys4) Rabbit mAb

 
Catalog #
ABZ5297
Application
WB, IF, FC, IP, ICC, IHC-P
Reactivity
Human, Mouse, Rat
Isotype
IgG
 
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  • Western blot analysis of MethylHistone H3 (di K4) in HeLa lysates using DiMethyl-Histone H3 (Lys4) antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human liver using MethylHistone H3 (di K4) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABZ5297-100 2 weeks 100 µL $499.00
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ABZ5297-20 2 weeks 20 µL $149.00
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Product Overview

NameDiMethyl-Histone H3 (Lys4) Rabbit mAb
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeIgG
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Calculated MW: 15 kDa; Observed MW: 15 kDa
Buffer System
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:1000; IHC-P-1:200-1:1000; ICC/IF-1:500-1:1000; FC-1:20-1:100; IP-1:20-1:50
Purification
Affinity Chromatography
Conjugation
Unconjugated
Modification
Dimethylated
Form
Liquid
Background
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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