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Human NMS(Neuromedin S) ELISA Kit

Human NMS(Neuromedin S) ELISA Kit

 
Catalog #
EKEL19851HU
Species
Human
Sensitivity
53.4 pg/mL
Assay Range
156.25-10000 pg/mL
Sample type(s)
Serum, plasma, other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL19851HU-96 2 weeks 96T $575.00
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EKEL19851HU-48 2 weeks 48T $400.00
EKEL19851HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman NMS(Neuromedin S) ELISA Kit
Accession (Primary)Q5H8A3
Reaction Duration2h
Target SpeciesHuman
ReactivityHuman
Sensitivity53.4 pg/mL
Assay Range156.25-10000 pg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, other biological fluids
Other Names
NM-S
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NMS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NMS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NMS in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
10000.000.212
5000.000.386
2500.000.557
1250.000.857
625.001.156
312.501.511
156.251.715
0.002.211
Standard curve: The concentration of NMS (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant NMS and the recovery rates were calculated by comparing the measured value to the expected amount of NMS in samples.

MatrixRecovery rangeAverage
Serum (n=5)88-102%95%
EDTA plasma (n=5)83-98%90%
Heparin plasma (n=5)81-95%86%

Target Information: ( Q5H8A3 )

NameNMS
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