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Human OPTN(Optineurin) ELISA Kit

Human OPTN(Optineurin) ELISA Kit

 
Catalog #
EKEL19871HU
Species
Human
Sensitivity
0.058 ng/mL
Assay Range
0.16-10 ng/mL
Sample type(s)
Tissue homogenates, cell lysates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL19871HU-96 2 weeks 96T $575.00
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EKEL19871HU-48 2 weeks 48T $400.00
EKEL19871HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman OPTN(Optineurin) ELISA Kit
Accession (Primary)Q96CV9
Reaction Duration3.5h
Target SpeciesHuman
ReactivityHuman
Sensitivity0.058 ng/mL
Assay Range0.16-10 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates, cell lysates and other biological fluids
Other Names
NRP; FIP2; GLC1E; HIP7; HYPL; TFIIIA-INTP; Glaucoma 1, Open Angle, E(Adult-Onset); Huntingtin-interacting protein 7; Optic neuropathy-inducing protein; E3-14.7K-interacting protein
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
10.001.9501.863
5.001.5271.440
2.501.2251.138
1.250.8720.785
0.630.5070.420
0.320.3050.218
0.160.1290.042
0.000.0870.000
Standard curve: The concentration of OPTN (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant OPTN and the recovery rates were calculated by comparing the measured value to the expected amount of OPTN in samples.

MatrixRecovery rangeAverage
Serum (n=5)90-105%97%
EDTA plasma (n=5)95-107%101%
Heparin plasma (n=5)78-90%84%

Target Information: ( Q96CV9 )

NameOPTN
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