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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL19894HU-96 | 2 weeks | 96T | $575.00 |
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| EKEL19894HU-48 | 2 weeks | 48T | $400.00 | |||
| EKEL19894HU-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Human FGF13(Fibroblast Growth Factor 13) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession (Primary) | Q92913 | |||||||||||||||||||||||||||
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Human | |||||||||||||||||||||||||||
| Reactivity | Human | |||||||||||||||||||||||||||
| Sensitivity | 3.2 pg/mL | |||||||||||||||||||||||||||
| Assay Range | 7.82-500 pg/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| FHF2; Fibroblast Growth Factor Homologous Factor 2 | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGF13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGF13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGF13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGF13 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant FGF13 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF13 in samples.
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Target Information: ( Q92913 ) | ||||||||||||||||||||||||||||
| Name | FGF13 | |||||||||||||||||||||||||||
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ADMEbio Team
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