| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL19940MU-96 | 2 weeks | 96T | $575.00 |
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| EKEL19940MU-48 | 2 weeks | 48T | $400.00 | |||
| EKEL19940MU-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Mouse MEP1a(Meprin A Alpha) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession (Primary) | P28825 | |||||||||||||||||||||||||||
| Reaction Duration | 2h | |||||||||||||||||||||||||||
| Target Species | Mouse | |||||||||||||||||||||||||||
| Reactivity | Mouse | |||||||||||||||||||||||||||
| Sensitivity | 19.1 pg/mL | |||||||||||||||||||||||||||
| Assay Range | 62.5-4000 pg/mL | |||||||||||||||||||||||||||
| Assay type | Competitive Inhibition | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma, cell lysates, cell culture supernates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant MEP1a and the recovery rates were calculated by comparing the measured value to the expected amount of MEP1a in samples.
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Target Information: ( P28825 ) | ||||||||||||||||||||||||||||
| Name | MEP1a | |||||||||||||||||||||||||||
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ADMEbio Team
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ADMEbio Team