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Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit

Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit

 
Catalog #
EKEL19958HU
Species
Human
Sensitivity
32 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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EKEL19958HU-96 2 weeks 96T $575.00
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EKEL19958HU-48 2 weeks 48T $400.00
EKEL19958HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit
Reaction Duration3.5h
Target SpeciesHuman
ReactivityHuman
Sensitivity32 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.002.2662.184
2500.001.7901.708
1250.001.2711.189
625.000.8470.765
312.500.4760.394
156.250.2980.216
78.130.2260.144
0.000.0820.000
Standard curve: The concentration of LAB7-1 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant LAB7-1 and the recovery rates were calculated by comparing the measured value to the expected amount of LAB7-1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)84-97%90%
EDTA plasma (n=5)87-95%91%
Heparin plasma (n=5)78-90%84%
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