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Chicken PGC(Pepsinogen C) ELISA Kit

Chicken PGC(Pepsinogen C) ELISA Kit

 
Catalog #
EKEL19995CK
Species
Chicken
Sensitivity
0.117 ng/mL
Assay Range
0.32-20 ng/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL19995CK-96 2 weeks 96T $735.00
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EKEL19995CK-48 2 weeks 48T $515.00
EKEL19995CK-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameChicken PGC(Pepsinogen C) ELISA Kit
Accession (Primary)Q9PWK1
Reaction Duration3.5h
Target SpeciesChicken
ReactivityChicken
Sensitivity0.117 ng/mL
Assay Range0.32-20 ng/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
PG-C; Progastricsin; Gastricsin; Pepsinogen II; Pepsinogen C
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken PGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken PGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken PGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken PGC in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
20.002.0271.924
10.001.3941.291
5.001.1161.013
2.500.7950.692
1.250.5890.486
0.630.3880.285
0.320.2240.121
0.000.1030.000
Standard curve: The concentration of PGC (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant PGC and the recovery rates were calculated by comparing the measured value to the expected amount of PGC in samples.

MatrixRecovery rangeAverage
Serum (n=5)90-105%97%
EDTA plasma (n=5)86-99%92%
Heparin plasma (n=5)79-93%86%

Target Information: ( Q9PWK1 )

NamePGC
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