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Human ES(Endostatin) ELISA Kit

Human ES(Endostatin) ELISA Kit

 
Catalog #
EKEL20037HU
Species
Human
Sensitivity
1.72 ng/mL
Assay Range
6.25-400 ng/mL
Sample type(s)
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20037HU-96 2 weeks 96T $575.00
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EKEL20037HU-48 2 weeks 48T $400.00
EKEL20037HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman ES(Endostatin) ELISA Kit
Reaction Duration2h
Target SpeciesHuman
ReactivityHuman
Sensitivity1.72 ng/mL
Assay Range6.25-400 ng/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Other Names
FLJ27325; FLJ34914; KNO; KNO1; MGC74745
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ES in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
400.000.195
200.000.467
100.000.612
50.000.997
25.001.236
12.501.557
6.251.889
0.002.232
Standard curve: The concentration of ES (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant ES and the recovery rates were calculated by comparing the measured value to the expected amount of ES in samples.

MatrixRecovery rangeAverage
Serum (n=5)81-97%89%
EDTA plasma (n=5)84-97%90%
Heparin plasma (n=5)82-94%90%
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