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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL20076HU-96 | 2 weeks | 96T | $575.00 |
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| EKEL20076HU-48 | 2 weeks | 48T | $400.00 | |||
| EKEL20076HU-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Human PNPLA2(Patatin Like Phospholipase Domain Containing Protein 2) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession (Primary) | Q96AD5 | |||||||||||||||||||||||||||
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Human | |||||||||||||||||||||||||||
| Reactivity | Human | |||||||||||||||||||||||||||
| Sensitivity | 0.133 ng/mL | |||||||||||||||||||||||||||
| Assay Range | 0.32-20 ng/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Tissue homogenates, cell lysates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| ATGL; PEDF-R; TTS2; Desnutrin; Pigment epithelium-derived factor; Transport-secretion protein 2; Adipose Triglyceride Lipase; Calcium-independent phospholipase A2 | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant PNPLA2 and the recovery rates were calculated by comparing the measured value to the expected amount of PNPLA2 in samples.
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Target Information: ( Q96AD5 ) | ||||||||||||||||||||||||||||
| Name | PNPLA2 | |||||||||||||||||||||||||||
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ADMEbio Team
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ADMEbio Team
