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Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit

Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit

 
Catalog #
EKEL20094HU
Species
Human
Sensitivity
0.96 ng/mL
Assay Range
3.13-200 ng/mL
Sample type(s)
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20094HU-96 2 weeks 96T $575.00
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EKEL20094HU-48 2 weeks 48T $400.00
EKEL20094HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
Reaction Duration2h
Target SpeciesHuman
ReactivityHuman
Sensitivity0.96 ng/mL
Assay Range3.13-200 ng/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Other Names
P3CP; C-Propeptide Of Type III Procollagen; Procollagen III Carboxy Terminal Propeptide
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
200.000.183
100.000.332
50.000.596
25.000.878
12.501.157
6.251.537
3.131.697
0.002.112
Standard curve: The concentration of PIIICP (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant PIIICP and the recovery rates were calculated by comparing the measured value to the expected amount of PIIICP in samples.

MatrixRecovery rangeAverage
Serum (n=5)83-98%91%
EDTA plasma (n=5)91-99%95%
Heparin plasma (n=5)82-95%92%
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